Confirming the identity of polysaccharides is becoming increasingly more important in the pharmaceutical, food, and specialty chemical industries. Creative Biolabs has established a powerful platform with a range of advanced technologies for polysaccharides analysis. We are confident in solving problems in polysaccharides analysis for our global customers.
Polysaccharides are often considered as a food source and structural building block. Their biological roles in many ways have been overlooked in the past due to their unique complexity. Nevertheless, over the last decade, researchers have increasingly paid their attention toward understanding the role of polysaccharides in normal cellular function and in disease, which opened up new research fronts in terms of probing glycans as targets in the design and development of novel drugs or new therapies for infectious disease, neurological disease, cancer, and metabolic disorders. Indeed, polysaccharides have been considered as novel important functional components for their significant biological activities, such as antioxidation, immune potentiation, antitumor, antiinflammation, and blood sugar reduction. The biological activities of polysaccharides are closely correlated to their physicochemical properties. Therefore, the characterization of polysaccharides is necessary.
Fig.1 Analysis of the primary structure of polysaccharides. (Ren, 2019)
Polysaccharides are very intricate complex, so the process of analysis is also manifold. Generally, monosaccharides composition and their linkage positions and sequence should be determined first. Then, the anomeric configuration of linkages, the ring size (furanose or pyranose), the absolute configuration (D or L), and any other substituents present should be identified. Up to now, there is no single method that can complete polysaccharide alone. Usually, separation and extraction techniques, component analysis, methylation analysis, glycosidic hydrolysis, mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy are required to determine the fine structure while size exclusion chromatography (SEC) coupled with light scattering (LS) detectors to determine the molecular size distribution.
Due to the intricate condition of polysaccharides, there is no single method capable of determining the total exact structure. Based on our top technology platforms, Creative Biolabs provides a series of polysaccharides analysis services to meet customers' specific requirements. Our seasoned scientists are extremely professional in the determination of molecular weight and structure of polysaccharides. Particularly, we will give you a satisfactory result based on our powerful technology platform.
SEC is used routinely to study the molecular weight and structural characteristics of macromolecules, and this technology is useful for polysaccharide characterization. It is a sophisticated technology available to provide information about the whole molecular weight distribution. SEC remains a popular molecular determination and separation method due to its versatility, the wide range of matrices commercially available, and the mild conditions of operation. One of the major advantages of SEC is that separation can be performed under conditions specifically designed to maintain the stability and activity of the molecule of interest without compromising resolution. With years of experience in SEC, Creative Biolabs is devoted to meeting any requirements of customers.
From NMR experiments of the native sample, structural features including the presence of deoxy or amino-deoxy sugars, the configuration of the glycosidic linkages and the ring size of the sugar residues, and the presence of non-sugar constituents, may be deduced. Due to less complexity of the spectra and better resolution, NMR has been widely applied to fractions obtained from partial degradation. MS methods may be applied to polymers as the matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) or pyrolysis-direct chemical ionization-MS. MS methods may also be applied to isolated oligomers or even the oligomeric mixtures. Structure information can be improved by recording the daughter ion spectra of single ions (Tandem-MS). Finally, the sequence of a polysaccharide chain could be reconstructed from the information gathered. Creative Biolabs has an excellent technical team and systematic technical platform to perfectly solve your trouble.
Fig.2 NMR analysis of pulp in ionic liquid electrolyte. (Holding, 2016)
Creative Biolabs is a forward-looking company that has a variety of advanced technologies and experienced scientists. Strong technical support makes us confident in providing the best service to any requirements. Please feel free to contact us for more information.