Quantitative Human C4 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0325-HX78)

Product Description	The quantitative human C4 (glycosylated) sandwich ELISA kit is designed to detect human complement 4 (C4) protein levels. It is suitable for various biological samples such as tissue homogenates, serum, or plasma. Its sensitivity is 0.421 ng/mL, which can accurately detect low concentrations of C4 in the sample.
Target	C4
N-Glycosylation Site	226, 862, 1328, 1391
O-Glycosylation Site	1244
Sample Types	Tissue homogenates, serum, plasma
Sample Volume	50 μL-100 μL
Sensitivity	0.421 ng/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	0.42 ng/mL-300 ng/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Species	Human
Full Name	Complement 4
Alternate Names	Acidic C4; Acidic complement C4; Basic C4; Basic complement C4; C3 and PZP-like α-2-macroglobulin domain-containing protein 2; C4; C4A anaphylatoxin; C4A; C4A2; C4A3; C4A4; C4A6; C4B; C4B1; C4B2; C4B3; C4F; C4S; Chido form of C4; CO4; Complement C4 A; Complement C4 B; Complement C4 gamma chain; Complement component 4A; Complement component 4B; Complement component 4F; Complement component 4S; CPAMD2; CPAMD3; Rodgers form of C4
Uniprot No.	P0C0L4

Application	The quantitative human C4 (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of C4, helping to understand its role in immune responses and inflammatory diseases.
Kit Components	Pre-coated 96-well ELISA plate; Lyophilized standard; HRP-conjugated C4 antibody; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 80-90%; Plasma sample: n=4, 90-100%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.