Quantitative Human CD274 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0325-HX109)

Product Description	The quantitative human CD274 (glycosylated) sandwich ELISA kit is designed to detect human programmed death ligand-1 (CD274) levels. It is suitable for various biological samples such as tissue homogenates, serum, or plasma. Its sensitivity is 3.904 pg/mL, which can accurately detect low concentrations of CD274 in the sample.
Target	CD274
N-Glycosylation Site	35, 192, 200, 219
Sample Types	Tissue homogenates, serum, plasma
Sample Volume	50 μL-100 μL
Sensitivity	3.904 pg/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	15.60 pg/mL-1000 pg/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Species	Human
Full Name	Programmed death ligand-1, CD274 molecule
Alternate Names	CD274 antigen; CD274 molecule; MGC142294; MGC142296; PD-L1; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; PDL1; Programmed death ligand 1; B7 homolog 1; B7-H1; B7H; B7H1; CD274
Uniprot No.	Q9NZQ7

Application	The quantitative human CD274 (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of CD274, helping to understand its role in tumor immune escape and related diseases.
Kit Components	Pre-coated 96-well ELISA plate; Lyophilized standard; Biotinylated CD274 antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 85-105%; Plasma sample: n=4, 90-110%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.