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Quantitative Human CD274 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0325-HX109

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Specifications Target Information Application Notes Related Products
Specifications
Product Description
The quantitative human CD274 (glycosylated) sandwich ELISA kit is designed to detect human programmed death ligand-1 (CD274) levels. It is suitable for various biological samples such as tissue homogenates, serum, or plasma. Its sensitivity is 3.904 pg/mL, which can accurately detect low concentrations of CD274 in the sample.
Target
CD274
Kit Components
Pre-coated 96-well ELISA plate; Lyophilized standard; Biotinylated CD274 antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
50 μL-100 μL
Sensitivity
3.904 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
15.60 pg/mL-1000 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Programmed death ligand-1, CD274 molecule
Alternate Names
CD274 antigen; CD274 molecule; MGC142294; MGC142296; PD-L1; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; PDL1; Programmed death ligand 1; B7 homolog 1; B7-H1; B7H; B7H1; CD274
Uniprot No.
Q9NZQ7
N-Glycosylation Site
35, 192, 200, 219
Application Notes
Application
The quantitative human CD274 (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of CD274, helping to understand its role in tumor immune escape and related diseases.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-105%;
Plasma sample: n=4, 90-110%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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