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Quantitative Human COL4α1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX152)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human COL4α1 (glycosylated) sandwich ELISA kit is designed to detect human collagen type 4 α1 (COL4α1) levels. COL4α1 is a major component of basement membranes, providing structural support and playing a critical role in filtration and barrier functions in various tissues. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 2.271 ng/mL, which can accurately detect low concentrations of COL4α1 in the sample.
Target COL4α1
N-Glycosylation Site 126
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 2.271 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 5.25 ng/mL-300 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Collagen type 4 α1
Alternate Names COL4α1; Collagen type 4 α1; COL4-A1; Collagen α1(IV) chain
Uniprot No. P02462
Application The quantitative human COL4α1 (glycosylated) sandwich ELISA kit is utilized in research investigating kidney diseases, Alport syndrome, and other basement membrane-related disorders. Researchers employ this ELISA to quantify COL4α1 levels, providing insights into its role in basement membrane integrity and disease pathogenesis.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-90%;
Plasma sample: n=4, 80-105%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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