Quantitative Human CUBN (Glycosylated) Sandwich ELISA Kit

The quantitative human CUBN (glycosylated) sandwich ELISA kit is designed to detect human cubilin (CUBN) levels. CUBN is a large peripheral membrane protein that functions as an endocytic receptor for various ligands, including vitamin B12-intrinsic factor complex, albumin, and apolipoprotein A-I. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.114 ng/mL, which can accurately detect low concentrations of CUBN in the sample.

CUBN

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.114 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-15 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Cubilin

CUBN; Cubilin; IFCR; GP280; Intestinal intrinsic factor receptor

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The quantitative human CUBN (glycosylated) sandwich ELISA kit is utilized to measure the concentration of glycosylated Cubilin in biological samples. This is essential for research investigating renal function, vitamin B12 metabolism, and protein reabsorption. The quantitative data obtained from this kit is valuable for understanding Cubilin's role in various physiological and pathological conditions, including kidney diseases.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 90-105%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.