Quantitative Human CUBN (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX71)

Product Description	The quantitative human CUBN (glycosylated) sandwich ELISA kit is designed to detect human cubilin (CUBN) levels. CUBN is a large peripheral membrane protein that functions as an endocytic receptor for various ligands, including vitamin B12-intrinsic factor complex, albumin, and apolipoprotein A-I. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.114 ng/mL, which can accurately detect low concentrations of CUBN in the sample.
Target	CUBN
N-Glycosylation Site	105, 428, 482, 711, 749, 781, 857, 957, 984, 1092, 1168, 1217, 1285, 1307, 1319, 1332, 1500, 1551, 1646, 1802, 1819, 1885, 2085, 2117, 2274, 2386, 2400, 2531, 2581, 2592, 2610, 2813, 2923, 2945, 3042, 3103, 3125, 3165, 3268, 3283, 3290, 3295, 3357, 3430, 3457, 3533, 3576
Sample Types	Tissue homogenates, cell lysates
Sample Volume	100 μL
Sensitivity	0.114 ng/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	0.55 ng/mL-15 ng/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Species	Human
Full Name	Cubilin
Alternate Names	CUBN; Cubilin; IFCR; GP280; Intestinal intrinsic factor receptor
Uniprot No.	O60494

Application	The quantitative human CUBN (glycosylated) sandwich ELISA kit is utilized to measure the concentration of glycosylated Cubilin in biological samples. This is essential for research investigating renal function, vitamin B12 metabolism, and protein reabsorption. The quantitative data obtained from this kit is valuable for understanding Cubilin's role in various physiological and pathological conditions, including kidney diseases.
Kit Components	Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 90-105%; Plasma sample: n=4, 80-95%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.