Quantitative Human CYP1A2 (Glycosylated) Sandwich ELISA Kit

The quantitative human CYP1A2 (glycosylated) sandwich ELISA kit is designed to detect human cytochrome P450 1A2 (CYP1A2) levels. CYP1A2 is a key enzyme in the metabolism of various drugs and xenobiotics, including caffeine and certain aromatic amines. It plays a significant role in both detoxification and activation of procarcinogens. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.361 ng/mL, which can accurately detect low concentrations of CYP1A2 in the sample.

CYP1A2

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.361 ng/mL

Quantitative sandwich ELISA

2 ng/mL-40 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Cytochrome P450 1A2

CYP1A2; Cytochrome P450 1A2; CP12; Cytochrome P450, family 1, subfamily A, polypeptide 2

69

The quantitative human CYP1A2 (glycosylated) sandwich ELISA kit is valuable in research investigating drug metabolism, pharmacogenetics, and the effects of environmental pollutants. Researchers employ this ELISA to quantify CYP1A2 levels, providing insights into individual variations in drug response and potential toxicological risks.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.