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Quantitative Human FAS (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX331

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Specifications
Product Description
The quantitative human FAS (glycosylated) sandwich ELISA kit is designed to detect human factor related apoptosis (FAS) levels. FAS is a transmembrane protein, which initiates apoptosis. Apoptosis is a programmed cell death process. FAS plays a crucial role in regulating immune responses. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.140 ng/mL, which can accurately detect low concentrations of FAS in the sample.
Target
FAS
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.140 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-20 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Factor related apoptosis
Alternate Names
FAS; Factor related apoptosis; CD95; ALPS1A; FAS1; TNFRSF6; Fas receptor; Tumor necrosis factor receptor superfamily member 6
Uniprot No.
N-Glycosylation Site
118, 136, 250
O-Glycosylation Site
28
Application Notes
Application
The quantitative human FAS (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated FAS levels. It is useful for studying the role of FAS in programmed cell death.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 95-110%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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