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Quantitative Human FUCα1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX162)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human FUCα1 (glycosylated) sandwich ELISA kit is designed to detect human fucosidase α-L1 (FUCα1) levels. FUCα1 is a lysosomal enzyme that hydrolyzes α-L-fucose residues from glycoproteins and glycolipids, playing a crucial role in the degradation of complex carbohydrates. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.142 ng/mL, which can accurately detect low concentrations of FUCα1 in the sample.
Target FUCα1
N-Glycosylation Site 241, 268, 382
Sample Types Tissue homogenates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.142 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-20 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Fucosidase α-L1
Alternate Names FUCα1; Fucosidase α-L1; α-L-Fucosidase
Uniprot No. P04066
Application The quantitative human FUCα1 (glycosylated) sandwich ELISA kit is valuable in research and clinical settings for diagnosing and monitoring fucosidosis, as well as investigating the role of FUCα1 in other metabolic disorders. Researchers employ this ELISA to quantify FUCα1 levels, providing insights into its activity and potential diagnostic or therapeutic applications.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-100%;
Plasma sample: n=4, 90-105%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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