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Quantitative Human HCN4 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX914)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human HCN4 (glycosylated) sandwich ELISA kit is designed to detect human hyperpolarization activated cyclic nucleotide gated potassium channel 4 (HCN4) levels. HCN4 is an ion channel protein that is primarily expressed in the heart's sinoatrial node, which is the heart's natural pacemaker. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.130 ng/mL, which can accurately detect low concentrations of HCN4 in the sample.
Target HCN4
N-Glycosylation Site 458
Sample Types Tissue homogenates
Sample Volume 100 μL
Sensitivity 0.130 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Hyperpolarization activated cyclic nucleotide gated potassium channel 4
Alternate Names HCN4; Hyperpolarization activated cyclic nucleotide gated potassium channel 4
Uniprot No. Q9Y3Q4
Application The quantitative human HCN4 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated HCN4 levels in biological samples. The assay is valuable for investigating the role of HCN4 in cardiac functions, arrhythmias, and neurological disorders.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 95-110%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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