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Quantitative Human IL25 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX803)

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1×48 T
1×96 T

Product Description The quantitative human IL25 (glycosylated) sandwich ELISA kit is designed to detect human interleukin 25 (IL25) levels. IL25 is a cytokine belonging to the IL-17 family. It is produced by various immune cells and epithelial cells. IL25 plays a role in promoting type 2 immune responses, which is involved in regulating inflammation and allergic reactions. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 7.024 pg/mL, which can accurately detect low concentrations of IL25 in the sample.
Target IL25
N-Glycosylation Site 136
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 7.024 pg/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 22.75 pg/mL-950 pg/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Interleukin 25
Alternate Names IL25; Interleukin 25; IL17E; Interleukin-17E
Uniprot No. Q9H293
Application The quantitative human IL25 (glycosylated) sandwich ELISA kit is used to determine IL25 levels in various experimental samples. It is valuable for research focused on understanding the roles of IL-25 in immune responses. This kit applies to studies of allergic inflammation, asthma research, and investigations of type 2 immunity.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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