Quantitative Human NCAN (Glycosylated) Sandwich ELISA Kit

The quantitative human NCAN (glycosylated) sandwich ELISA kit is designed to detect human neurocan (NCAN) levels. NCAN is a chondroitin sulfate proteoglycan primarily expressed in the central nervous system, playing a crucial role in regulating neuronal plasticity, cell adhesion, and extracellular matrix organization. It is involved in processes such as axonal growth, synapse formation, and glial cell function. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.253 ng/mL, which can accurately detect low concentrations of NCAN in the sample.

NCAN

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates

100 μL

0.253 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-15 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Neurocan

NCAN; Neurocan; NEUR; Neurocan core protein; Chondroitin sulfate proteoglycan 3

122, 340, 1026, 1223

381, 411

The quantitative human NCAN (glycosylated) sandwich ELISA kit is valuable for researchers investigating neurological disorders, such as brain injury, stroke, and neurodegenerative diseases, where NCAN expression and function are altered. By quantifying NCAN, researchers can gain insights into its role in disease pathogenesis and explore potential diagnostic or therapeutic applications related to neuronal repair and regeneration.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-105%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.