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Quantitative Human NCAN (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX26)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human NCAN (glycosylated) sandwich ELISA kit is designed to detect human neurocan (NCAN) levels. NCAN is a chondroitin sulfate proteoglycan primarily expressed in the central nervous system, playing a crucial role in regulating neuronal plasticity, cell adhesion, and extracellular matrix organization. It is involved in processes such as axonal growth, synapse formation, and glial cell function. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.253 ng/mL, which can accurately detect low concentrations of NCAN in the sample.
Target NCAN
N-Glycosylation Site 122, 340, 1026, 1223
O-Glycosylation Site 381, 411
Sample Types Tissue homogenates
Sample Volume 100 μL
Sensitivity 0.253 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Neurocan
Alternate Names NCAN; Neurocan; NEUR; Neurocan core protein; Chondroitin sulfate proteoglycan 3
Uniprot No. O14594
Application The quantitative human NCAN (glycosylated) sandwich ELISA kit is valuable for researchers investigating neurological disorders, such as brain injury, stroke, and neurodegenerative diseases, where NCAN expression and function are altered. By quantifying NCAN, researchers can gain insights into its role in disease pathogenesis and explore potential diagnostic or therapeutic applications related to neuronal repair and regeneration.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-105%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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