Quantitative Human OPN (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0325-HX832)

Product Description	The quantitative human OPN (glycosylated) sandwich ELISA kit is designed to detect human osteopontin (OPN) levels. It is suitable for various biological samples such as tissue homogenates, serum, plasma, or urine. Its sensitivity is 0.224 ng/mL, which can accurately detect low concentrations of OPN in the sample.
Target	OPN
O-Glycosylation Site	134, 138, 143, 147, 152, 234, 308
Sample Types	Tissue homogenates, serum, plasma, urine
Sample Volume	50 μL-100 μL
Sensitivity	0.224 ng/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	0.85 ng/mL-50 ng/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Species	Human
Full Name	Secreted phosphoprotein 1
Alternate Names	Secreted phosphoprotein 1; Spp-1; Osteopontin; Bone sialoprotein 1; Calcium oxalate crystal growth inhibitor protein; SPP-1; OPN
Uniprot No.	P10451

Application	The quantitative human OPN (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of OPN, helping to understand its role in cancer.
Kit Components	Pre-coated 96-well ELISA plate; Lyophilized standard; Biotinylated OPN antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 80-100%; Plasma sample: n=4, 95-110%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.