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Quantitative Human P4HTM (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX854)

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1×48 T
1×96 T

Product Description The quantitative human P4HTM (glycosylated) sandwich ELISA kit is designed to detect human transmembrane prolyl 4-hydroxylase (P4HTM) levels. P4HTM is an enzyme that is a subunit of prolyl 4-hydroxylase. This enzyme catalyzes the formation of 4-hydroxyproline in collagen. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 6.024 pg/mL, which can accurately detect low concentrations of P4HTM in the sample.
Target P4HTM
N-Glycosylation Site 348, 368, 382
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 6.024 pg/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 20 pg/mL-900 pg/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Transmembrane prolyl 4-hydroxylase
Alternate Names P4HTM; Transmembrane prolyl 4-hydroxylase; EGLN4; FLJ20262; HIF-prolyl hydroxylase 4; HIFPH4; Hypoxia-inducible factor prolyl hydroxylase 4; P4HTM_HUMAN; Proline 4 hydroxylase; Prolyl hydroxlase domain containing 4
Uniprot No. Q9NXG6
Application The quantitative human P4HTM (glycosylated) sandwich ELISA kit is used to measure P4HTM levels in biological samples. This kit is useful in studies examining collagen synthesis and fibrosis. It is also relevant to studies investigating the role of P4HTM in the formation of the extracellular matrix and the development of connective tissue disorders.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 90-110%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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