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Quantitative Human RANk (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX943)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human RANk (glycosylated) sandwich ELISA kit is designed to detect human receptor activator of nuclear factor kappa B (RANk) levels. RANk is a transmembrane protein. RANk is expressed on osteoclasts, which binds to its ligand, RANKL, which is expressed on osteoblasts. This interaction is crucial for osteoclast differentiation. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.074 ng/mL, which can accurately detect low concentrations of RANk in the sample.
Target RANk
N-Glycosylation Site 105, 174
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.074 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Receptor activator of nuclear factor kappa B
Alternate Names RANk; Receptor activator of nuclear factor kappa B; CD265; TNFRSF11A; ODFR; Osteoclast differentiation factor receptor
Uniprot No. Q9Y6Q6
Application The quantitative human RANk (glycosylated) sandwich ELISA kit is used to measure RANK levels in biological samples. The assay enables the study of RANK's role in bone metabolism, osteoporosis, and related skeletal diseases.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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