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Quantitative Human RPN1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX174)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human RPN1 (glycosylated) sandwich ELISA kit is designed to detect human ribophorin 1 (RPN1) levels. RPN1 is a subunit of the N-oligosaccharyltransferase (OST) complex, essential for the cotranslational N-glycosylation of proteins in the endoplasmic reticulum. It plays a crucial role in protein folding and quality control. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.791 ng/mL, which can accurately detect low concentrations of RPN1 in the sample.
Target RPN1
N-Glycosylation Site 299
Sample Types Tissue homogenates
Sample Volume 100 μL
Sensitivity 0.791 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 2.5 ng/mL-100 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Ribophorin 1
Alternate Names RPN1; Ribophorin 1; OST1; RBPH1; Dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit 1
Uniprot No. P04843
Application The quantitative human RPN1 (glycosylated) sandwich ELISA kit is valuable in research investigating protein glycosylation, endoplasmic reticulum stress, and diseases related to glycosylation defects. Researchers utilize this ELISA to quantify RPN1 levels, providing insights into its role in protein processing and cellular function.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 90-110%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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