Quantitative Rat ADAM17 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX925)

Product Description	The quantitative rat ADAM17 (glycosylated) sandwich ELISA kit is designed to detect rat a disintegrin and metalloprotease 17 (ADAM17) levels. ADAM17 is a transmembrane protein that is responsible for the shedding of various cell surface proteins. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.094 ng/mL, which can accurately detect low concentrations of ADAM17 in the sample.
Target	ADAM17
N-Glycosylation Site	157, 264, 452, 498, 539, 551, 606
Sample Types	Tissue homogenates, cell lysates
Sample Volume	100 μL
Sensitivity	0.094 ng/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	1 ng/mL-10 ng/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Species	Rat
Full Name	A disintegrin and metalloprotease 17
Alternate Names	ADAM17; A disintegrin and metalloprotease 17; CSVP; Disintegrin and metalloproteinase domain-containing protein 17
Uniprot No.	Q9Z1K9

Application	The quantitative rat ADAM17 (glycosylated) sandwich ELISA kit is used to measure ADAM17 levels in biological samples. The assay enables the study of ADAM17's role in cytokine processing, cell signaling, and inflammatory processes.
Kit Components	Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 85-95%; Plasma sample: n=4, 80-90%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.