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Quantitative Rat ADAM17 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX925

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Specifications
Product Description
The quantitative rat ADAM17 (glycosylated) sandwich ELISA kit is designed to detect rat a disintegrin and metalloprotease 17 (ADAM17) levels. ADAM17 is a transmembrane protein that is responsible for the shedding of various cell surface proteins. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.094 ng/mL, which can accurately detect low concentrations of ADAM17 in the sample.
Target
ADAM17
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.094 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Rat
Full Name
A disintegrin and metalloprotease 17
Alternate Names
ADAM17; A disintegrin and metalloprotease 17; CSVP; Disintegrin and metalloproteinase domain-containing protein 17
Uniprot No.
N-Glycosylation Site
157, 264, 452, 498, 539, 551, 606
Application Notes
Application
The quantitative rat ADAM17 (glycosylated) sandwich ELISA kit is used to measure ADAM17 levels in biological samples. The assay enables the study of ADAM17's role in cytokine processing, cell signaling, and inflammatory processes.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-95%;
Plasma sample: n=4, 80-90%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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