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Quantitative Rat AFP (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0125-HX52

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Specifications Target Information Application Notes Related Products
Specifications
Product Description
The quantitative rat AFP (glycosylated) sandwich ELISA kit is designed to detect rat alpha-fetoprotein (AFP) protein levels. It is suitable for various biological samples such as tissue homogenates, serum, plasma, or cell culture supernates. Its sensitivity is 3.906 pg/mL, which can accurately detect low concentrations of AFP in the sample.
Target
AFP
Sample Types
Tissue homogenates, serum, plasma, cell culture supernates
Sample Volume
50 μL-100 μL
Sensitivity
3.906 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
15.62 pg/mL-1000 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Rat
Full Name
Alpha-fetoprotein
Alternate Names
Alpha-fetoprotein; Alpha-1-fetoprotein; AFP
Uniprot No.
P02773
N-Glycosylation Site
117, 253
Application Notes
Application
The quantitative rat AFP (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of AFP, helping to understand its role in cancer.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-95%;
Plasma sample: n=4, 90-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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