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Quantitative Rat C4 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0325-HX79

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Specifications
Product Description
The quantitative rat C4 (glycosylated) sandwich ELISA kit is designed to detect rat complement 4 (C4) protein levels. It is suitable for various biological samples such as tissue homogenates, serum, or plasma. Its sensitivity is 3.112 ng/mL, which can accurately detect low concentrations of C4 in the sample.
Target
C4
N-Glycosylation Site
224, 664, 743, 1323, 1386
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
50 μL-100 μL
Sensitivity
3.112 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
12.50 ng/mL-800 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Rat
Full Name
Complement 4
Alternate Names
Acidic C4; Acidic complement C4; Basic C4; Basic complement C4; C3 and PZP-like α-2-macroglobulin domain-containing protein 2; C4; C4A anaphylatoxin; C4A; C4A2; C4A3; C4A4; C4A6; C4B; C4B1; C4B2; C4B3; C4F; C4S; Chido form of C4; CO4; Complement C4 A; Complement C4 B; Complement C4 gamma chain; Complement component 4A; Complement component 4B; Complement component 4F; Complement component 4S; CPAMD2; CPAMD3; Rodgers form of C4
Uniprot No.
Application Notes
Application
The quantitative rat C4 (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of C4, helping to understand its role in immune responses and inflammatory diseases.
Kit Components
Pre-coated 96-well ELISA plate; Lyophilized standard; HRP-conjugated C4 antibody; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-100%;
Plasma sample: n=4, 85-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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