Recombinant Avian infectious bronchitis virus (strain D3896) Spike glycoprotein (S) (318-538 aa) [His-Tag], E. coli

Recombinant Avian infectious bronchitis virus (strain D3896) Spike glycoprotein (318-538 aa) was expressed in E. coli with a 6xHis-tag at the C-terminus. The biological activity was determined by its binding ability in a functional ELISA. This product can be used in ELISA, WB, IP.

Avian infectious bronchitis virus (strain D3896)

318-538 aa

ESDYMYGSYHPSCKFRLETINNGLWFNSLSVSLGYGPIQGGCKQSVFQNRATCCYAYSYNGPPLCKGVYRGELTKSFECGLLVFVTKTDGSRIQTRNEPFTLTQHNYNNITLDRCVEYNIYGRVGQGFITNVTNYAINYNYLADGGMAILDTSGAIDIFVVQGEYGLNYYKVNPCEDVNQQFVVSGGKLVGILTSRNETGSQPLENQFYIKIINGTRRSRR

6xHis-tag at the C-terminus

25.8 kDa

>90%, determined by SDS-PAGE.

Unconjugated

S

Spike glycoprotein

S1 attaches the virion to the cell membrane by interacting with cell receptors, initiating the infection. S2 is a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.

S glycoprotein; E4; Peplomer protein; S