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Recombinant Human Inter-alpha-trypsin inhibitor heavy chain 4 (NP_002209.2) (689-930 aa) was expressed in E. coli with a 6xHis-SUMO-tag at the N-terminus. The biological activity was determined by its binding ability in a functional ELISA. This product can be used in ELISA, WB, IP.
The protein is secreted into the blood, where it is cleaved by plasma kallikrein into two smaller forms. This protein has been detected only in liver, and it seems to be upregulated during surgical trauma.