Recombinant Infectious hematopoietic necrosis virus (strain Round Butte) Glycoprotein (21-508 aa), E. coli

Recombinant Infectious hematopoietic necrosis virus (strain Round Butte) (IHNV) Glycoprotein (21-508 aa) was produced using E. coli cell-free expression system with a His-tag or Tag free. Measured by its binding ability in a functional ELISA.

Measured by its binding ability in a functional ELISA.

E. coli cell-free expression system

Infectious hematopoietic necrosis virus (strain Round Butte) (IHNV)

21-508 aa

His-tag or Tag free

>90%, determined by SDS-PAGE.

Unconjugated

G

Glycoprotein

Binds to specific receptor at cellular surface, bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the viral nucleocapsid into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induce an irreversible conformational change in G, releasing a fusion hydrophobic peptide.

Spike glycoprotein; G