Recombinant Mokola virus (MOKV) Glycoprotein G (20-522 aa), E. coli

Recombinant Mokola virus (MOKV) Glycoprotein G (20-522 aa) was produced using E. coli cell-free expression system with a His-tag or Tag free. Measured by its binding ability in a functional ELISA.

Measured by its binding ability in a functional ELISA.

E. coli cell-free expression system

Mokola virus (MOKV)

20-522 aa

His-tag or Tag free

>90%, determined by SDS-PAGE.

Unconjugated

G

Glycoprotein G

Attaches the virus to host cellular receptor, inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. There is convincing in vitro evidence that the muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) bind glycoprotein and thereby facilitate rabies virus entry into cells.

Glycoprotein G