Recombinant SARS-CoV-2 Surface glycoprotein (S) (319-541 aa) [His/Myc-Tag], Mammalian cell

Recombinant Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Surface glycoprotein (319-541 aa) was expressed in Mammalian cell with a 10xHis-tag at the N-terminus and a a Myc-tag at the C-terminus. The biological activity was determined by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 3 μg/mL can bind SARS-CoV-2-S antibody, the EC50 of SARS-CoV-2-S1-RBD protein is 22.14 ng/mL. This product can be used in ELISA, WB, IP.

Determined by its binding ability to SARS-CoV-2-S antibody in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 3 μg/mL can bind SARS-CoV-2-S antibody, the EC50 of SARS-CoV-2-S1-RBD protein is 22.14 ng/mL.

Mammalian cell

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

319-541 aa

RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF

10xHis-tag at the N-terminus and Myc-tag at the C-terminus

30.1 kDa

>95%, determined by SDS-PAGE.

<1.0 EU/μg, determined by the LAL method.

Unconjugated

S

Surface glycoprotein

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.

Spike glycoprotein