Creative Biolabs is a world-leading service provider in glycoprotein analysis services for global customers based on our most comprehensive and advanced technical platform. Our service program covers every step of glycoprotein analysis from glycan release to glycan identification. We have developed a series of technologies for glycan analysis, from which you can choose an optimal analytical strategy to meet your project.

Technical Background of Glycoprotein Analysis

The presence, absence, and profile of the complex oligosaccharide moieties on the biopharmaceuticals, such as monoclonal antibodies (mAbs), have an essential effect on their therapeutic efficacy, pharmacokinetics, immunogenicity, folding, and stability. To better analysis of glycoform and glycomics, most techniques have been developed. Typical glycoprotein analysis techniques include lectin microarray, mass spectrometry (MS), high-performance liquid chromatography (HPLC) on various solid phases. MS is coupled with different separation methods, such as capillary electrophoresis (CE) and liquid chromatography (LC) used for glycoprotein analysis. Some detection methods are also used for glycan analysis such as pulsed amperometric detection (PAD) and fluorescence detection (FLD). The following picture shows the common methods applied for glycoprotein analysis.

Glycan Analysis Services at Creative Biolabs

The selection of appropriate methods for a specific glycoprotein is determined by the purpose of detection and the capacity of individual technique in analyzing intact glycoproteins, glycopeptides, released glycans, and monosaccharides. With years of experience in glycoproteins analysis, Creative Biolabs has established a variety of technical platforms for glycoprotein analysis including number and location of N- and O-linked glycosylation sites identification, the degree of glycosylation site occupancy detection, characterization of oligosaccharide structure, sialic acid content and type, etc. We are confident in offering comprehensive glycoprotein analysis services for global customers based on our diverse technical platforms. Our glycoprotein analysis services include Glycoprotein Detection, Glycoprotein Structure Analysis, and Glycoprotein Quantification. Besides, our excellent experts can also provide detailed technical guidance and help our customers formulate an optimal analysis protocol to accelerate the later research.

Techniques Advantages in Glycan Analysis
Lectin Microarray
  • Compared to the MS-based approach, it directly works on intact glycoproteins without the need for the liberation of glycans.
  • It enables us to offer rapid and high-sensitivity profiling of complex glycan features.
MS
  • MS in conjugation with different separation methods is the most powerful tool for analysis of glycan types and glycosylation levels/alterations.
  • Several tandem MS methods are also used for glycopeptide characterization, such as CE-MA and MALDI-MS.
LC-ESI-MS
  • Determination of intact protein mass.
  • Glycoform profiling of more complex glycoproteins.
MALDI-TOF MS
  • Rapid glycopeptide profiling and glycans profiling.
  • Excellent speed, low sample consumption, high throughput, and automation potential.
GC-MS
  • Primarily used for monosaccharide and linkage analysis of glycans.
  • Detection of pM amount of samples.
HPLC
  • Used for the isolation and analysis of both glycoproteins and their derived carbohydrates.
  • Excellent resolution, ease of use, the generally high recoveries, the excellent reproducibility of repetitive separations, and the high productivity in terms of cost parameters.
HPAEC-PAD
  • Analyzing both monosaccharides and all classes of oligosaccharides without derivatization.
RP-HPLC
  • Used for analysis and purification of peptides, proteins, and glycoproteins.
  • Low picomolar amounts of resolved components can be collected.
UHPLC/FLD/Q-TOF
  • Good glycans release from therapeutic glycoproteins.
  • Sensitive and quantitative analysis of the labeled glycans.
  • High confidence identification of even minor glycoforms and isoforms.
FTIR
  • High-resolution data over a wide spectral range in a short time.
  • Non-invasive and label-free.
NMR
  • Nondestructive, leaving the sample intact for further analysis.
  • Small sample amounts.
SPRi
  • Label-free interaction analysis.
  • Real-time monitoring of kinetic and affinity parameters.
TLC
  • Both plant- and animal-derived carbohydrates.
  • Both neutral and acidic monosaccharide, oligosaccharide and polysaccharide.
Flow Cytometry
  • Lectin based-flow cytometry analysis. Hundreds of lectins for choice, such as LCA, PNA, SNA and LEL, etc.
  • Diverse cells can be analyzed, including tumor cells, immune cells and other cell lines.

Why Choose Us

  • Diverse samples are available, including glycoproteins, carbohydrates, cell lines and other complicated samples
  • Whole-process technical trace service
  • High-sensitivity and high-resolution analytical platform
  • High flexibility and cost-effectiveness

Equipped with various technologies and experienced expert staff, Creative Biolabs is proud to offer the most comprehensive glycan analysis. Over the past several years, Creative Biolabs wins high praise from all over the world. We believe that our best services will promote your project success. If you are interested in our services, please don’t hesitate to contact us for more information and a detailed quote. If you have some puzzles in glycoprotein analysis, let us know and we will assist you.

Reference

  1. Zhang, L.; et al. Glycan analysis of therapeutic glycoproteins. Mabs. 2016, 8(2):205-215.
For lab research only.

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