Introduction of GPR15
GPR150 belongs to the G-protein-coupled receptor (GPCR) family which is cylindrical receptor protein usually found in the cellular membrane and is involved in signal transduction, by which chemical messengers found outside of the cell can alter intra-cellular protein activity and gene expression. The main structural characteristic of GPR150 is seven transmembrane α-helices (TMs). GPR150 is encoded by the GPR150 gene which is located at 5q15. Besides, GPR150 is comprised of 434 amino acids in human, and the mass of GPR150 is 46,353 Da.
|Basic Information of GPR150|
|Protein Name||Probable G-protein coupled receptor 150|
|Organism||Homo sapiens (Human)|
Function of GPR150 Membrane Protein
The human GPR150 was found in a sequence submitted to the nr database already in 2001 but not described as a GPCRs. In fact, GPR150 is one member of G-protein coupled receptor 1 family, and it is found to be related to the β-group of Rhodopsin family. The main bioactivity of GPR150 is mediated by G protein. Besides, GPR150 is found in mammals only, which means the receptor arise lately. The closest relatives of the GPR150 are the oxytocin and arginine-vasopressin receptors (OXTR/AVPR) which are involved in water balance, it is possible that the function of the GPR150 is essential only for land-living species. What’s more, methylated promoter CpG islands (CGIs) can be used to find novel tumor-suppressor genes and disease markers. It has been proved that, in primary ovarian cancers, CGIs of GPR150 (in 4 of 15 cancers) were methylated, so aberrant methylation of GPR150 could be candidate tumor markers.
Fig.1 The G-protein activation/deactivation cycle (Thomsen, 2005).
Application of GPR150 Membrane Protein in Literature
This article discusses the appeal of neuropeptide-GPCRs systems as drug targets and provides an overview of concepts in the evolution of vertebrate genomes and gene families. And GPR150 is one of the drug targets.
This study reveals the extent of constitutive activity among orphan class A G-protein coupled receptors within the cAMP signaling pathway. The activity of GPR150 is inhibited under both baseline and forskolin stimulated.
This article reveals that CGIs of GPR150 (in 4 of 15 cancers), ITGA8 (2/15), PRTFDC1 (1/15), and HOXD11 (1/15) are methylated in primary ovarian cancers. Silencing of PRTFDC1 is revealed here for the first time, and aberrant methylation of GPR150, ITGA8 and HOXD11 could be candidate tumor markers.
This article reports nine new members of the Rhodopsin family of human G protein-coupled receptors (GPCRs) including GPR150 found by searches in the genome databases.
GPR150 Preparation Options
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