From individuals to exogenous agents, such as drugs, vaccines, immune responses are affected by the human leukocyte antigen (HLA) tissue type. The variation of HLA genes takes an essential role in HLA’s function in regulating the immune response. Many diseases are also associated with specific HLA types. In the field of hematopoietic stem cell transplantation (HSCT), the selection of donors includes a rigorous assessment of the HLA match status to the recipient. Hence, it will be of great importance for the researchers to make an accurate and precise interpretation of the HLA genes information through HLA typing. If you are conducting a clinical trial, establishing the tissue type of your donor sample is also a key step. And bringing your test results together with the sample HLA type will provide you the most valuable explanation of the experiment.
Creative Biolabs provides you the most outstanding and highly accurate HLA tissue typing service, which meets specific requirements in sequencing resolution, time, budget, and research objectives. Based on the advanced technical background and rich clinical experience, our comprehensive ECIA™ HLA tissue typing Service offers serological methods, PCR-SSO, PCR-SSP, PCR-SBT, and NGS technologies to help customers acquire complicated HLA allele information for both academic research and clinical application.
ECIA™ HLA tissue typing service offers a mature and efficient technical system, which consists of sequence-specific oligonucleotide (PCR-SSO) technology, sequence-specific primer (PCR-SSP) technology, sequencing-based typing (PCR-SBT) and the next-generation sequencing (NGS). Compared to traditional serological typing, these methods have better accuracy and sensitivity.
Sequence-specific oligonucleotide (PCR-SSO): The isotopic or non-radiolabeled probe is used to hybridize the target fragment resulted from PCR amplification to determine the individual genotype based on the positive spot. A variety of different probes are firstly immobilized on the same membrane, and the PCR product is in turn hybridized to the probe after labeled. This method has the advantages of high sensitivity, specificity, and low sample size.
Sequence-specific primer (PCR-SSP): HLA-specific amplification products are obtained by PCR and analyzed by electrophoresis to determine the HLA type. This method is very useful and cost-effective for clinical application.
Sequencing-based typing (PCR-SBT): Based on the sequencing results of gene polymorphism regions, this method could provide characteristics the HLA alleles with high precision.
The next-generation sequencing (NGS): Combined with clonal amplification, this method could provide phase information and sequence larger regions of genes, and has been proved to be a cost-efficient HLA typing strategy.
Fig.1 Structure of MHC (HLA) I and II Molecules.