Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
Highlights| Protein Name | Histamine receptor H4 |
| Gene Name | HRH4 |
| Uniprot | Q9H3N8 (Human); Q91ZY2 (Mouse) |
| Synonym | H4; H4R; BG26; HH4R; AXOR35; GPRv53; GPCR105; histamine H4 receptor; G-protein coupled receptor 105; SP9144; pfi-013 |
| Background | Histamine H4 receptor (H4R or HRH4), is an integral membrane protein which in human is encoded by HRH4 gene. It is recognized as a histamine receptor belonging to the family of rhodopsin-like G-protein-coupled receptors. HRH4 is predominantly expressed in bone marrow and shows generally restricted expression in immune cells. It is also expressed in the liver, colon, lung, spleen, small intestine, testes, tonsils, thymus, and trachea. It is also found in the cerebellum and hippocampus. The expression of HRH4 is either up-regulated or down-regulated upon activation of the lymphoid tissues and this regulation may rely on the presence of IL10/interleukin-10 or IL13/interleukin-13. |
Creative Biolabs offers comprehensive range of membrane protein products empowers your unique research needs.
| CAT# | Product Name | Expression System | Protein Length | Solubilizing Agents |
| S01YF-1023-KX181 | NativeExtract™ Human HRH4 Membrane Protein (Full length, Super Nanodisc) | HEK293 cells | Full length | Native Nanodisc |
| MPX3520K | MemDX™ Membrane Protein Human HRH4 (Histamine receptor H4) Expressed in vitro E.coli expression system, Full Length | E.coli cell-free | Full length | Detergent |
| Please for more products or customization | ||||
L**y: I recently ordered a protein from Creative Biolabs, and I’m really satisfied! The product arrived quickly and was exactly as described. It worked perfectly in my experiments. The customer service was also very helpful. Highly recommend!
11/ Jun/2023
R**y: The team was super responsive to my questions. Would definitely order again!
28/ Jul/2023
We provide tailored solutions to meet your specific requirements. Please inquire us for more services.
Creative Biolabs offers gene editing services. Advanced gene editing platforms are increasingly instrumental in the development of HRH4 knockout cellular lines and corresponding animal models, thereby facilitating in-depth investigations into HRH4 function. The precise genomic targeting essential for such gene ablation necessitates the meticulous design and synthesis of specific guide RNAs (gRNAs) engineered to direct the endonuclease machinery to the HRH4 locus. Subsequent to the gene editing process, rigorous validation of knockout efficiency is imperative.
Creative Biolabs offers AAV mediated gene delivery services. These sophisticated viral constructs are meticulously engineered to deliver the HRH4 gene, or its specific allelic variants, to designated cellular or tissue targets. Critically, the utility of such AAV-based delivery systems can be significantly enhanced. It is often feasible to incorporate tissue-specific promoter elements within these vectors, a refinement that permits the controlled expression of the HRH4 transgene predominantly within desired anatomical compartments or specific cell lineages in vivo, thereby achieving a high degree of precision in gene regulation.
Creative Biolabs offers siRNA and shRNA knockdown services. The targeted suppression of HRH4 expression in pertinent cell lines was achieved using RNA interference (RNAi) technologies, such as small interfering RNAs (siRNAs) or vector-based short hairpin RNAs (shRNAs). Subsequent meticulous validation of the extent of HRH4 silencing is, of course, critical. This confirmation is typically achieved by quantifying residual HRH4 mRNA levels, commonly via qPCR, and by assessing the concomitant reduction in HRH4 protein abundance through Western blot analysis.
Creative Biolabs offers comprehensive and innovative services to drive the development of HRH4 cell therapy. Please inquire with us for more services.
Creative Biolabs offers generation of HRH4-overexpressing cell lines services. Stable transfection is employed to engineer cell lines for the sustained and heritable expression of the HRH4, facilitating the introduction of the HRH4 gene construct into appropriate host cellular systems. The subsequent selection and isolation of clonal cell lines exhibiting robust and high-level HRH4 expression then commonly rely upon the co-transfection and utility of specific selectable markers, which confer a survival advantage under defined culture conditions.
Creative Biolabs offers generation of HRH4-modified immune cell services. To interrogate the immunomodulatory functions of the HRH4, viral vector systems are commonly utilized to engineer its expression within key immune cell subsets, including pivotal populations. Such analyses typically assess the impact of enforced HRH4 expression on a spectrum of diverse cellular responses—for instance, alterations in proliferative capacity, the repertoire and magnitude of secreted cytokines, and the efficiency of cytotoxic effector mechanisms.
Creative Biolabs offers stem cell engineering services. A sophisticated approach to investigating the functional significance of the HRH4 involves its targeted introduction into pluripotent stem cells (PSCs), such as embryonic or induced pluripotent stem cells; these genetically modified PSCs are subsequently directed to differentiate along specific pathways into desired somatic cell lineages. This experimental framework provides an invaluable platform. It facilitates detailed interrogation of HRH4's potential contributions to the intricate molecular and cellular mechanisms governing differentiation, and further allows for an assessment of its influence on endogenous tissue regeneration capabilities.
Creative Biolabs has worked all out in the field of membrane protein preparation to support membrane protein studies. We are recognized as the world leader in providing the largest and most diverse portfolio of standard and custom professional membrane protein preparation services. Please feel free to for professional technical support.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
