Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsWith over a decade of experience in phage display technology, Creative Biolabs can provide a series of antibody or peptide libraries that are available for licensing or direct screening. These ready-to-use libraries are invaluable resources for isolating target-specific binders for various research, diagnostic or therapeutic applications. Highlights
Creative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
HighlightsYeast surface displayed human proteome libraries have been used to identify protein fragments with affinity for various target molecules, including phosphorylated peptides. When combined with fluorescently activated cell sorting and high throughput methods for the analysis of selection outputs, yeast surface displayed human proteome libraries can rapidly and efficiently identify protein fragments with high affinity for any soluble ligand that can be fluorescently detected, especially for posttranslational modifications. Based on decades of experience and advanced technology, Creative Biolabs has constructed large libraries that display human protein fragments for identifying posttranslational modification-dependent protein interactions.
Identification of Posttranslational Modification-Dependent Protein Interactions
The posttranslational modifications (PTMs) play a critical role in signal transmission or functional regulation for many cellular processes. Over 200 types of PTM are generated by thousands of cellular enzymes, including phosphorylation, glycosylation, lipidation, sumoylation, acetylation, ubiquitination, and methylation (not limited). The identification of proteins that interact specifically with posttranslational modifications such as phosphorylation is usually essential to understand cellular signaling pathways.
Identification of Posttranslational Modification-Dependent Protein Interactions via Yeast Display
Yeast display, as a eukaryotic surface display system, is a new technique that allows direct affinity capture using modified ligands and ready identification of the binding protein based on a tight linkage between the protein and its encoding gene. Yeast human proteome display libraries provide a flexible and powerful tool for identifying protein fragments with affinity for most soluble target ligand and are particularly suitable for the study of PTM-dependent protein interactions. Since it has been demonstrated that yeast protein expression pathways are similar to those found in mammalian cells, human protein fragments displayed by yeast human proteome libraries are perfectly possible to be properly folded and functional. Compared with protein microarrays, yeast displayed human proteome libraries can be generated relatively quickly at low cost and are an easily renewable resource. When combined with a compatible method of high throughput nucleic acid analysis (e.g. exon microarrays or next-generation sequencing), selection outputs can be comprehensively screened, allowing the discovery of a greater diversity of interactions.
Why Choose Us?
At Creative Biolabs, our scientists have constructed large libraries that display human protein fragments (around 100-400 amino acids) on the yeast surface as C-terminal fusions to the yeast a-agglutinin subunit, Aga2p and successfully applied to identify protein fragments that bind to post-translationally modified phosphorylated peptides, small signaling molecule phosphatidylinositides, and monoclonal antibodies. With years of experience, Creative Biolabs can provide services in identifying posttranslational modification-dependent protein interactions using yeast surface displayed human proteome libraries for our worldwide clients.
If you are interested in learning more about Creative Biolabs’ yeast display services, please contact us for more details. We also offer other Yeast Display Library Screening services: Premade Human Antibody Library for Yeast Display.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
