4T1 In Vitro Cell Membrane Integrity-based Apoptosis Assay (FCM)

This assay is to provide 4T1-based In Vitro Cell Membrane Integrity-based Apoptosis Assay (FCM) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

4T1

Mouse

4T1 is a breast cancer cell line derived from the mammary gland tissue of a mouse BALB/c strain. 4T1 cells are epithelial and are resistant to 6-thioguanine. In preclinical research, 4T1 cells have been used to study breast cancer metastasis as they can metastasize to the lung, liver, lymph nodes, brain and bone.

Breast Cancer

Oncology

In Vitro Cell Membrane Integrity-based Apoptosis Assay (FCM)

4T1-cell based In Vitro Cell Membrane Integrity-based Apoptosis Assay (FCM)

FCM is one of the best and accurate automated tecqniques used for the quantification of apoptosis. FCM has been widely used in cancer drug discovery approaches to detect apoptosis. FCM can be used to monitor integrity of cancer cell membranes. Dyes such as PI, fluorescein diacetate, carboxyfluorescein diacetate and TO-PRO-3 are commonly used examples for this purpose in FCM.

Detection of Apoptosis Assays

Apoptosis (programmed cell death) plays a vital role in embryonic development, homeostasis, functioning of immune system and wound repair. The ability to evade induction of apoptosis has been used by cancer cells to survive against host defense mechanisms. The molecular mechanisms involved in cancer cell apoptosis have been well documented and it involves certain biochemical events such as DNA fragmentation, chromatin condensation, cell organelle degradation and protein cleavage, etc. The extrinsic and intrinsic (mitochondrial) pathways are the two major pathways involved in apoptosis. With the available techniques and assays, a number of apoptosis inducing agents (natural compounds, synthetic compounds, nano-formulations, peptides and enzymes) in many cancer cells have been identified. Selection of an assay for apoptosis detection is based on factors such as apoptotic pathway, nature of drug, cell type being used and the method of analysis.

Flow Cytometry to Detect Apoptosis Assays

Flow Cytometry Assay