4T1 In Vitro Matrix Metalloproteinase Activity Assay (Angiogenesis)

This assay is to provide 4T1-based In Vitro Matrix Metalloproteinase Activity Assay (Angiogenesis) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

4T1

Mouse

4T1 is a breast cancer cell line derived from the mammary gland tissue of a mouse BALB/c strain. 4T1 cells are epithelial and are resistant to 6-thioguanine. In preclinical research, 4T1 cells have been used to study breast cancer metastasis as they can metastasize to the lung, liver, lymph nodes, brain and bone.

Breast Cancer

Oncology

In Vitro Matrix Metalloproteinase Activity Assay (Angiogenesis)

4T1-cell based In Vitro Matrix Metalloproteinase Activity Assay (Angiogenesis)

Matrix metalloproteinases (MMPs) belong to a family of proteases (consist of 24 known human proteases), which can proteolytically degrade ECM protein. Angiogenesis involves the formation of new blood vessels, degradation and remodeling of the ECM to permit endothelial cell migration and invasion into
the neighboring tissue. MMPs have thus been considered in involving remodeling of ECM in angiogenesis. Several MMPs have been identified to enhance angiogenesis by forming promigratory ECM components, responsible for the cleavage of endothelial cell-cell adhesions and release of angiogenic growth factors bound to ECM. MMP activities are modified at transcription level, by endogenous inhibitors and by tissue inhibitors of metalloproteinases. Different assay approaches based on immunochemical and enzyme activities have been introduced by several manufacturers to assess MMP activity at cellular level.

Angiogenesis Monitoring Assays

Local and regional metastasis is a life-threatening condition in cancer, which is hard to control with the available cancer treatment options. In metastasis, cancer cells move away from primary tumors and travel through the blood and lymphatic system and form new tumors on other parts. A vascular
network growth is important for cancer metastasis. Angiogenesis is a process that is known as the formation of new blood vessels with the help of existing blood vessels and angiogenesis has been reported to play a major role in cancer growth and metastasis. Tumors can send chemical signals to nearby normal tissues to stimulate angiogenesis. New blood vessels can feed growing tumors with nutrients and oxygen, allowing cancer cells to spread (metastasis).