4T1 In Vitro S-nitrosylation Assay (Oxidative Stress)

This assay is to provide 4T1-based In Vitro S-nitrosylation Assay (Oxidative Stress) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

4T1

Mouse

4T1 is a breast cancer cell line derived from the mammary gland tissue of a mouse BALB/c strain. 4T1 cells are epithelial and are resistant to 6-thioguanine. In preclinical research, 4T1 cells have been used to study breast cancer metastasis as they can metastasize to the lung, liver, lymph nodes, brain and bone.

Breast Cancer

Oncology

In Vitro S-nitrosylation Assay (Oxidative Stress)

4T1-cell based In Vitro S-nitrosylation Assay (Oxidative Stress)

S-nitrosylation (attachment of NO to thiol side chains of the amino acid cysteine) and S-glutathionylation (attachment of GSH to thiol side chains of the amino acid cysteine) are two post-translational modification processes reported to increase with oxidative stress.

Oxidative Stress Assays

Disturbance between the production of reactive oxygen species (ROS), free radicals and antioxidant mechanisms is defined as the oxidative stress, or more precisely, it is an imbalance between the oxidant and antioxidant state in cells. This imbalance can cause harmful effects to cells and biomolecules, which ultimately causes adverse effects in the whole organism. Oxidative imbalance can target important proteinsand lipids in cells, which can increase the risk of developing a cancer. On the other hand, increased ROS production in cancer cells by certain cancer drugs can also arrest cancer cell cycle and cause senescence and apoptosis through oxidative stress.

Markers Thiobarbituric Acid Reactive Substances