4T1 In Vitro Spheroid Migration Assay (Migration)

This assay is to provide 4T1-based In Vitro Spheroid Migration Assay (Migration) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

4T1

Mouse

4T1 is a breast cancer cell line derived from the mammary gland tissue of a mouse BALB/c strain. 4T1 cells are epithelial and are resistant to 6-thioguanine. In preclinical research, 4T1 cells have been used to study breast cancer metastasis as they can metastasize to the lung, liver, lymph nodes, brain and bone.

Breast Cancer

Oncology

In Vitro Spheroid Migration Assay (Migration)

4T1-cell based In Vitro Spheroid Migration Assay (Migration)

Cell spheroids are attached to a surface of cell culture wells or dishes and are allowed to migrate to another region of the wells where cells start to attach and form a new monolayer. This assay resembles 2D and 3D culturing technique and mimics an in vivo system. Cells that form spheroids can only be used for this assay and inclusion of two different types of cells (co- cultivation) to monitor invasion is possible with this assay. Fluorescence and light microscopy can be used to monitor invasion of cells.

Cell Migration and Invasion Assays

The movement of cancer cells and formation of tumors in neighboring tissue is a sequential cascade known as invasion-metastatic cascade, which includes cancer cell invasion through extracellular matrix (ECM), intravasation into blood and colonization at sites after they exit from blood circulation. Migration and invasion are two different processes where, cell migration involves movement of cells without passing through a barrier such as ECM, whereas cell invasion involves passing through a barrier such as ECM while destroying it.

Cell Migration Assay

Cell Migration Assay