Assay Name
In Vitro Reporter Gene (β-galactosidase) Assay
Short Description
9L-cell based In Vitro Reporter Gene (β-galactosidase) Assay
Assay Description
Reporter gene assays have become a valuable technique for studying gene expression by both cis- and trans-acting regulatory factors. The action of a reporter gene is evaluated in a reporter gene assay and a reporter gene construct normally comprises of a target regulatory DNA sequence (usually a major in a cancer pathway) to be analyzed, sequence for the reported gene and a sequence for functional mRNA in an expression vector. The expression vector can be transfected into the desired cell type. After transfection of the vector, the effects on a drug on the expression of target gene can be evaluated. The ideal reported gene activity should detect with high sensitivity over any endogenous gene expression. Reporter gene systems permit the use of cancer pathway-specific or developmentally regulated regulatory DNA sequences as markers to analyze gene expression in specific pathways associated with molecular mechanisms of cancer.
Assay Reporter
β-galactosidase
Assay Type
High-throughput Screening, High Content Screening and Reporter Gene Assays
Datasheet