A549 In Vitro BrdU Assay (Cell Proliferation)

CAT#: ITS-1022-YF1694
Target Cell Organism: Human
Target Cell Name: A549
Assay Type: Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Overview
This assay is to provide A549-based In Vitro BrdU Assay (Cell Proliferation) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
A549
Target Cell Organism
Human
Target Cell Background
A549 cells were isolated from the lung tissue of a White, 58-year-old male with lung cancer. This cell line can be used in cancer, immuno-oncology and toxicology research.
Related Diseases
Lung Carcinoma
Research Area
Oncology
Assay Name
In Vitro BrdU Assay (Cell Proliferation)
Short Description
A549-cell based In Vitro BrdU Assay (Cell Proliferation)
Assay Description
5-Bromo-2'-deoxyuridine (BrdU) assay is another methodological approach used to determine cell proliferation. BrdU incorporated into synthesized DNA can be determined using BrdU-specific monoclonal antibody. A secondary antibody is used to generate fluorescent signal on DNA synthesis.
Assay Alternative Names
5-Bromo-2'-deoxyuridine (BrdU) Assay
Assay Type
Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Type Details
Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.
Assay Subtype
Cell Proliferation Assay
Assay MOA
Cell-based Assays Based on DNA Synthesis
For Research Use Only | Not For Clinical Use
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