Six-reagent kit for staining and visualization of glomerular proteins in kidney biopsies. A.F.O.G. kit is used for staining kidney biopsies. It can be used instead of P.A.S.M. kit because it consists of Aniline Blue, Orange G and Acid Fuchsin combination of dyes.
If the kit is used with tissues fixed in formalin, muscle tissues are stained red instead of green.
Result Nuclei - dark blue to black Muscle fibers - green (red if the tissue is fixed with formalin) Basal membrane - cyclamen color. Fibrin, erythrocytes - hues ranging from yellow to red. Connective fibers - blue.