Antibody & Protein Production Service
Introduction Our Services Publication Why Choose Us FAQs Customer Review Related Services Contact Us
Creative Biolabs has over a decade of experience in therapeutic protein drug discovery and development. They offer a comprehensive, end-to-end service portfolio—including antibody discovery, engineering, production, characterization, and in vivo testing—to accelerate projects to application faster.
Driving Innovation in Life Sciences
The production of high-quality antibodies and recombinant proteins is a cornerstone of modern biomedical research and therapeutic development. These biomolecules are the fundamental tools for diagnostics, drug discovery, and clinical applications. Advancements in genetic immunization and recombinant protein technology have overcome many traditional limitations, allowing for the generation of antibodies against previously intractable targets, such as membrane proteins. This is critical for advancing fields like immuno-oncology and structural biology, as demonstrated by numerous scientific publications and the high success rates achieved by specialized service providers like Creative Biolabs.
Here is the general information about the advantages of four systems of heterologous protein expression for
recombinant protein production. The more stars there are, the better the corresponding standpoint is.
(Kind reminder: Results may vary from one protein to another)
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Bacteria
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Yeast
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Insect
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Mammalian cells
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The Cornerstone of Scientific Research: High-Purity Protein Production
Recombinant proteins are indispensable tools across a vast range of applications, from structural biology studies and enzyme assays to cell signaling research and vaccine development. However, achieving high yields of functional, correctly folded proteins can be a significant technical hurdle. A poorly expressed or impure protein can compromise experimental results and delay critical timelines.
Creative Biolabs leverages a suite of state-of-the-art expression platforms to overcome these challenges. Our comprehensive protein production services span all major host systems, including:
Bacterial Expression System (E. coli)
Ideal for producing small, non-glycosylated proteins with high yields and at a low cost.
Yeast Expression System
A robust system for expressing secreted proteins and complex, disulfide-bonded molecules.
Baculovirus/Insect Cell Expression System
Perfect for producing large, complex proteins with post-translational modifications, offering high expression levels and scalability.
Mammalian Cell Expression System
The gold standard for producing complex therapeutic proteins and antibodies, ensuring native folding, glycosylation, and biological activity.
Publication
This publication addresses the challenge of generating antibodies for membrane proteins, which is often limited by the availability of purified or natively folded antigens. The study describes a genetic immunization approach that uses DNA alone to produce antibodies against a diverse set of bacterial and viral membrane proteins. They successfully produced antibodies for 71% of the 17 targets studied.
To facilitate antibody characterization, the researchers also developed a new in vitro method, called IVT-HMB, for the simultaneous expression and capture of membrane protein targets. This method simplified antigen preparation by eliminating the need to purify detergent-solubilized or urea-denatured membrane proteins. The antibodies generated from this approach were used to characterize the expression and membrane-targeting of several F. tularensis proteins, demonstrating antibody specificity. The authors conclude that these methods will accelerate future structural and functional studies of therapeutically relevant membrane proteins.
Fig.1 Development of high-specificity polyclonal antibodies against membrane proteins.1
Why Choose Us?
Our deep scientific expertise and flexible, client-focused approach are what set Creative Biolabs apart. We are pioneers in cutting-edge techniques like genetic immunization, which allows us to successfully produce antibodies for challenging targets like multi-pass membrane proteins—a common roadblock for many researchers. Our commitment to quality and innovation ensures that your project is not only successful but also completed with unparalleled efficiency and scientific rigor. Our ability to deliver high-quality, functionally active proteins and antibodies where others have failed is a testament to our specialized platforms and decades of collective experience.
Highlights:
Comprehensive
A series of expression platforms including bacteria, yeast, insect, and mammalian cells.
Guaranteed quality
Based on our advanced facilities and technologies.
End-to-end solutions
A complete pipeline from gene synthesis, vector construction, pilot testing, to large-scale production.
Custom service
Offer customized service to ensure your flexible choice is executed efficiently.
Experience the Creative Biolabs Advantage - Get a Quote Today!
FAQs
Q1: How do you ensure the quality and purity of the final protein or antibody?
A1: Our process includes a multi-stage quality control protocol. After purification, we perform rigorous characterization using techniques like SDS-PAGE, western blotting, ELISA, and mass spectrometry to confirm purity, concentration, and functionality. We won't proceed to the next step until we are fully confident in the quality of the product.
Q2: Can you produce difficult targets like multi-pass membrane proteins?
A2: Yes, this is one of our key strengths. We have developed specialized platforms, including genetic immunization and our in-house in vitro expression system, specifically to address the challenges associated with producing these complex targets. We invite you to contact us to discuss your specific membrane protein project.
Q3: Can you produce complex proteins that require specific post-translational modifications like glycosylation?
A3: Yes, we can. For proteins requiring complex modifications like glycosylation, we recommend using our mammalian cell expression systems. These systems are equipped with the cellular machinery to produce human-like modifications, which are often critical for protein functionality, stability, and immunogenicity.
Customer Review
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High-quality Product
Using Creative Biolabs' recombinant protein production service in our research has significantly improved the efficiency of our in vitro assays. The high purity and correct folding of the protein were essential for our success. - M*** A
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Functional Biomolecules
Our drug screening initiative was stalled until Creative Biolabs provided a large batch of functionally active, correctly glycosylated signaling protein. The quality was exceptional and allowed us to complete a critical set of experiments on schedule. - P*** O
Related Services
To further support your research, we recommend exploring our complementary services:
Enzyme Inhibition Assessment Service
Creative Biolabs provides enzyme inhibition services to assess drug-drug interactions. By identifying these interactions, we help you mitigate risks, optimize dosing, and ensure superior patient outcomes, accelerating the path to a safer drug product.
Learn More →
In Vivo Tumor Lineage Tracing Services
Tumor lineage tracking is crucial for understanding cancer progression and identifying therapeutic targets. Creative Biolabs offers various in vivo tumor lineage tracing methods, including site-specific recombinant enzyme systems and barcodes. Our new, high-resolution techniques enable single-cell readout and high-resolution analysis of tumor evolution.
Learn More →
Contact Us
As a leading protein production solution provider, Creative Biolabs is committed to guiding our clients from gene to protein to ensure the optimal strategy to fit the target protein features within budget and timeline. If you have antibody or protein production requirements, please contact us for more information and a detailed quote.
Reference
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Hansen, Debra T., et al. "Polyclonal antibody production for membrane proteins via genetic immunization." Scientific reports 6.1 (2016): 21925. Distributed under Open Access license CC BY 4.0, without modification. DOI: https://doi.org/10.1038/srep21925
