Azan Trichrome kit (CAT#: ITS-1022-ZP38)

Five-reagent kit for connective tissue staining according to Mallory. Used for visualization of muscle fibers, collagen, glial cells, glomerular cells and erythrocytes.

The Azan Trichrome kit is a modified version of the Mallory Trichrome kit for connective tissue staining. It is used to visualize muscle, collagen fibers, glial cells, glomerular cells, chromatin, and erythrocytes from the same section. The kit contains two acid dyes: Azo-Carmine G and Aniline Blue Counterstain. Azocarmine G was used at the beginning of the staining procedure and aniline blue was used at the final stage after the sections were treated with phosphomolybdic acid. In order to obtain high-quality staining results, it is necessary to stain the sections with azocarmine G, followed by stepwise differentiation with an aniline alcohol solution to counterstain certain structures of the sections, such as collagen.

Tissue Stainning