B16F10 In Vitro Spheroid Migration Assay (Migration)

This assay is to provide B16F10-based In Vitro Spheroid Migration Assay (Migration) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

B16F10

Mouse

B16F10 cell line (from Mus musculus C57BL/6J strain) is a convenient and widely used experimental model of highly metastatic melanoma to study cytotoxicity, migration, metastatic spread and tissue invasion. B16-F10 is a cell line exhibiting a morphology of spindle-shaped and epithelial-like cells that was isolated from skin tissue of a mouse with melanoma. Use these cells in your skin cancer research.

B16-F10

Melanoma

Oncology

In Vitro Spheroid Migration Assay (Migration)

B16F10-cell based In Vitro Spheroid Migration Assay (Migration)

Cell spheroids are attached to a surface of cell culture wells or dishes and are allowed to migrate to another region of the wells where cells start to attach and form a new monolayer. This assay resembles 2D and 3D culturing technique and mimics an in vivo system. Cells that form spheroids can only be used for this assay and inclusion of two different types of cells (co- cultivation) to monitor invasion is possible with this assay. Fluorescence and light microscopy can be used to monitor invasion of cells.

Cell Migration and Invasion Assays

The movement of cancer cells and formation of tumors in neighboring tissue is a sequential cascade known as invasion-metastatic cascade, which includes cancer cell invasion through extracellular matrix (ECM), intravasation into blood and colonization at sites after they exit from blood circulation. Migration and invasion are two different processes where, cell migration involves movement of cells without passing through a barrier such as ECM, whereas cell invasion involves passing through a barrier such as ECM while destroying it.

Cell Migration Assay

Cell Migration Assay