C2BBe1 In Vitro RNA Expression Analysis Assay (Northern Blooting)

This assay is to provide C2BBe1-based In Vitro RNA Expression Analysis Assay (Northern Blooting) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

C2BBe1

Human

The C2BBe1 (brush border expressing) cell line was cloned in 1988 from the Caco-2 cell line by limiting dilution.
The clone was selected on the basis of morphological homogeneity and exclusive apical villin localization.

Colorectal Adenocarcinoma; Colon Cancer

Oncology

In Vitro RNA Expression Analysis Assay (Northern Blooting)

C2BBe1-cell based In Vitro RNA Expression Analysis Assay (Northern Blooting)

Northern blotting is a conventional method used to analyze RNA levels. In northern blotting, mRNA isolated from cells or tissues are quantified by gel electrophoresis and transferred to membranes and incubated with suitable probes. The probes are normally labeled with chemiluminescent substrates or radioactive isotopes. Bound RNA-probe complexes are visualized on X-ray films.

Gene and Protein Expression Assays

Following transcription, primary transcript RNA is converted into mature RNA by post-transcriptional modifications. Post-transcriptional modification includes three major steps namely the addition of a 5'cap, addition of a 3'polyadenylation tail and RNA splicing. Stability of RNA has been considered as very important for gene expression and proteins at the post-transcriptional level.