CAL 27 In Vitro (3)H-thymidine Incorporation Assay (Cell Proliferation)

This assay is to provide CAL 27-based In Vitro (3)H-thymidine Incorporation Assay (Cell Proliferation) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

CAL 27

Human

Cal 27 cells are epithelial cells isolated from tissue taken prior to treatment from a 56-year-old, White male with a lesion in the middle of the tongue in 1982.

Squamous Cell Carcinoma

Oncology

In Vitro (3)H-thymidine Incorporation Assay (Cell Proliferation)

CAL 27-cell based In Vitro (3)H-thymidine Incorporation Assay (Cell Proliferation)

The 3H-thymidine incorporation assay is one such assay that can detect newly synthesized DNA in cells. Cultured cells are mixed with 3H-thymidine (radioactive) and kept for a desired incubation period. Newly replicated DNA strands can incorporate 3H-thymidine during mitosis. A scintillation counter is necessary to detect the output. Direct measurement of proliferating cells is the main advantage of this assay. However, use of harmful radioactive substances and need of special equipment are considered as main disadvantages.

Cell Viability/Cytotoxicity and Antiproliferative Assays

Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.

Cell Proliferation Assay

Cell-based Assays Based on DNA Synthesis