The Calcium Stain Kit (Modified Von Kossa) is designed for the histological visualization of calcium deposits in paraffin or cryosections. The modified Von Kossa method is a precipitation reaction in which silver ions react with phosphate (instead of calcium) in the presence of an acid. Photochemical degradation of silver phosphate to silver then occurs under UV light irradiation. Tissue is treated with silver nitrate solution, silver replaces calcium reduced by strong light, so it can be regarded as metallic silver. To confirm the presence of calcium, nuclear fast red solution was used to confirm the presence of calcium deposits.
Features
Calcium in mass deposits: Black Calcium in dispersed deposits: Gray Nuclei: Red Cytoplasm: Light Pink
Applications
Tissue Stainning
Application Notes
Not to be taken internally. For In-Vitro DiaCreic use only. Histological applications. Do not use past expiration date. Use caution when handling reagents. Non-Sterile.
Components
Silver Nitrate Solution (5%) 125 ml 2-8°C Sodium Thiosulfate Solution (5%) 125 ml 18-25°C Nuclear Fast Red Solution 125 ml 18-25°C
Precaution of Use
Avoid contact with skin and eyes. Harmful if swallowed. Follow all federal, State, and local regulations regarding disposal. Use in chemical fume hood whenever possible.
Storage
Mixed storage conditions. Store according to individual label instructions.
