Cat IFNG ELISA Kit, Lot 21AO-1019 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P930
Product Type: ELISA Kit
Target: IFNG
Short Description
The kit is designed for in vitro quantitative measurement of Cat IFNG in Cell Culture Supernatant, Plasma, Serum.
Description
Feline IFN-gamma ELISA Kit for cell culture supernatants, plasma, and serum samples.
Applications
ELISA
Application Notes
Recommended Dilution for serum and plasma samples: 2-fold
Target
IFNG
Reactivity
Cat
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Cell Culture Supernatant, Plasma, Serum
Specificity
Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: feline Fas, IL-1 beta, IL-2, IL-4, IL-5, IL-8, IL-10, IL-12p40, RANTES
Components
Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate
Material not included
Distilled or deionized water
Precision pipettes to deliver 2 μL to 1 μL volumes
Adjustable 1-25 μL pipettes for reagent preparation
100 μL and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis
Sensitivity
0.24 ng/mL
Sample Volume
100 μL
Plate
Pre-coated
Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Note
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Restrictions
For Research Use only
Alternative Name
Interferon gamma
Synonyms
IFG; IFI; IFN-g; Ifg; IFNG2; IFN-gamma; IFN-G; IFNG; IFNgamma; TCRalpha; INF-G; ifng; interferon gamma; interferon; gamma 1-2; IFNG; Ifng; ifng1-2
Background
Gene Names: IFNG
Protein names: Interferon gamma (IFN-gamma)
Gene ID
493965
UniProt
P46402
Pathways
Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
Protocol
1.Prepare all reagents, samples and standards.
2.Add 100μL standard or sample to each well. Incubate 1 hours at 37 °C.
3.Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C.
4.Aspirate and wash 3 times.
5.Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C.
6.Aspirate and wash 5 times.
7.Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C.
8.Add 50μL Stop Solution. Read at 450nm immediately.
For Research Use Only | Not For Clinical Use
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