Cat IL2 ELISA Kit, Lot 21AO-685 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P596
Product Type: ELISA Kit
Target: IL2
Short Description
The kit is designed for in vitro quantitative measurement of Cat IL2 in Cell Culture Supernatant, Plasma, Serum.
Description
Feline IL-2 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Applications
ELISA
Application Notes
Recommended Dilution for serum and plasma samples: 2-fold
Target
IL2
Reactivity
Cat
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Cell Culture Supernatant, Plasma, Serum
Specificity
This ELISA antibody pair detects feline IL-2. Approximately 3 % cross-reactivity with recombinant canine IL-2 is observed, approximately 1 % cross reactivity with recombinant porcine IL-2 and recombinant human IL-2 is observed.
Components
Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate
Material not included
Distilled or deionized water
Precision pipettes to deliver 2 μL to 1 μL volumes
Adjustable 1-25 μL pipettes for reagent preparation
100 μL and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis
Sensitivity
25 pg/mL
Sample Volume
100 μL
Plate
Pre-coated
Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Note
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Restrictions
For Research Use only
Alternative Name
Interleukin 2
Synonyms
IL2; IL-2; il2; TCGF; lymphokine; Il-2; POIL2; interleukin 2; interleukin-15; IL2; il2; LOC100303688; Il2
Background
Gene Names: IL2
Protein names: Interleukin-2 (IL-2) (T-cell growth factor) (TCGF)
Gene ID
751114
UniProt
Q07885
Pathways
JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
Protocol
1.Prepare all reagents, samples and standards as instructed in the manual.
2.Add 100 μL of standard or sample to each well.
3.Incubate 2.5 h at RT or O/N at 4°C.
4.Add 100 μL of prepared biotin antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 μL of prepared Streptavidin solution to each well.
7.Incubate 45 min at RT.
8.Add 100 μL of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 μL of Stop Solution to each well.
11.Read at 450 nm immediately.
For Research Use Only | Not For Clinical Use
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