Luciferase Cell Line Products
A Luciferase Cell Line is a genetically engineered cell line designed to express the luciferase enzyme. This enzyme catalyzes a bioluminescent reaction, producing light in the presence of its substrate (commonly luciferin), and is widely used in research to monitor cellular activity. By tagging genes or proteins of interest with the luciferase marker, you can quantify biological processes, such as gene expression, protein interactions, and cell viability, through luminescence intensity. Luciferase cell lines are particularly valuable for high-throughput screening, real-time imaging, and in vitro and in vivo studies, offering a sensitive, non-invasive, and quantifiable method to analyze various cellular functions and responses.
Advantages of Luciferase Cell Lines
High Sensitivity
Highly sensitive, enabling detection of low-level biological activities or signals, making it ideal for studying weak gene expression or low-abundance proteins.
Real-Time Monitoring
Luminescence produced by luciferase can be monitored in real-time, allowing you to observe dynamic cellular processes continuously and non-invasively.
Quantitative Analysis
Luminescence intensity correlates with luciferase activity, providing a direct and quantifiable readout of gene expression, cellular response, or protein interactions.
Low Background Noise
Luciferase reactions have minimal background signal since mammalian cells lack endogenous luciferase, leading to clearer, more accurate results.
High Throughput Compatibility
The luciferase assay's simplicity and sensitivity make it compatible with high-throughput screening.
Broad Utility
Luciferase cell lines are versatile, suitable for various applications.
Non-Toxic and Non-Destructive
Luciferase expression and luminescence do not harm cells, enabling multiple time-point measurements on the same sample without disrupting cellular health.
Featured Products
| Cat. NO | Product Name | Optical Reporter | Quantity |
|---|---|---|---|
| IOC-ZP014 | CellOnco™ Human HepG2-Luc Reporter Tumor Cell Line | Luciferase | 1x10^6 cells/vial |
| IOC-02P033 | CellOnco™ Mouse CT26-Luc Tumor Cell Line | Luciferase | 1x10^6 cells/vial |
| IOC-ZP003 | CellOnco™ Mouse GL261 Luc Reporter Tumor Cell Line | Luciferase | 1x10^6 cells/vial |
| IOC-ZP129 | CellOnco™ Human NALM6-Luc Reporter Tumor Cell Line | Luciferase | 1x10^6 cells/vial |
| IOC-ZP059 | CellOnco™ Human U-251-Luc Reporter Tumor Cell Line | Luciferase | 1x10^6 cells/vial |
| IOC-ZP002 | CellOnco™ Human K562 Luc Reporter Tumor Cell Line | Luciferase | 1x10^6 cells/vial |
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Applications of Luciferase Cell Lines
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Gene Expression Studies
Allowing researchers to analyze promoter activity, gene regulation, and transcriptional responses to stimuli. -
Drug Screening and Development
Measure the impact of chemicals on target genes or pathways to enable high-throughput screening of possible therapeutic candidates. -
Cell Viability and Cytotoxicity Assays
Assess the cytotoxicity of drugs or environmental factors, providing insights into cellular health and apoptotic pathways. -
Signal Transduction Pathway Analysis
Monitor specific signaling pathways (e.g., NF-kB, CREB, or Wnt pathways), aiding in understanding how cells respond to various stimuli or drugs at the molecular level. -
Protein-Protein Interactions
Study protein interactions within cells, providing insights into complex cellular processes and identifying therapeutic targets.
Workflow of Luciferase Cell Lines
Published Data
Fig.1 The figure above shows the assay readout of luciferase in a single cell culture.1
Contact Us for Custom Luciferase Cell Lines
If you are interested in incorporating luciferase technology into your research or need a specific luciferase cell line tailored to your project requirements, we are here to help. As a trusted supplier of custom luciferase cell lines, we specialize in developing cell models optimized for sensitivity, stability, and efficiency to meet your unique experimental needs. For more information on our custom luciferase cell line offerings, or to discuss your project specifics, please contact us today. We look forward to supporting your research with high-quality, reliable luciferase cell lines designed to enhance your results.
Reference
- Mosaad, E.O et al. "Constraints to counting bioluminescence producing cells by a commonly used transgene promoter and its implications for experimental design." Sci Rep 9, 11334 (2019). Distributed under Open Access license CC BY 4.0, without modification.