Cell Tube Formation Assay Services
At Creative Biolabs, we specialize in providing comprehensive cell tube formation assay services tailored to evaluate angiogenesis. Our assay is a robust in vitro model that mimics cellular events during capillary formation, allowing for the screening of pro- and anti-angiogenic factors, drug efficacy, and the mechanistic understanding of vascular development. The assay involves culturing endothelial cells on a reconstituted basement membrane matrix, such as Matrigel, where they differentiate, migrate, and align to form capillary-like tubular structures.
Explore Cell Tube Formation Assay Services
Tumor Cell Tube Formation Assay
Tumor cells can influence angiogenesis by producing pro-angiogenic factors, an ability crucial for tumor growth and metastasis. The tumor cell tube formation assay is a specialized service designed to simulate the tumor microenvironment and examine the proangiogenic potential of tumor cells. This assay is pivotal for researchers seeking to understand the mechanisms of tumor angiogenesis and to evaluate the efficacy of anti-angiogenic therapies.
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Endothelial Cell Tube Formation Assay
Our endothelial cell tube formation assay is ideal for studying endothelial cell biology, screening pro- and anti-angiogenic compounds, and understanding the impact of genetic modifications on angiogenesis. The assay typically uses primary endothelial cells or cell lines, alongside potential pro-angiogenic or anti-angiogenic factors, to examine the effect on tube formation. Our assay involves the use of endothelial cells cultured on a reconstituted basement membrane matrix.
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Image Analysis of Tube Formation
To provide you with precise and comprehensive insights, Creative Biolabs employs a range of sophisticated technologies for endothelial cell tube formation assays. Our methodologies include several image analysis techniques to capture detailed aspects of tube formation:
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Calcein AM Labeling: This fluorescent dye allows for the live staining of endothelial cells, providing vibrant images that facilitate the quantification of tube structures and network formation.
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Fixation and Crystal Violet Staining: This method offers robust and permanent staining of endothelial tubes, facilitating detailed visualization under various imaging modalities.
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Light Microscopy: Utilizing high-resolution light microscopy, we deliver clear and detailed images of tube formation, enabling accurate analysis and interpretation of angiogenesis.
Application of Tube Formation Assays
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Endothelial Cell Functionality: Measure the inherent ability of endothelial cells to form capillary-like structures.
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Assess Pro-Angiogenic Factors: Determine the ability of diverse tumor cell lines to induce the formation of capillary-like structures.
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Screening Anti-Angiogenic Compounds: Evaluate potential therapeutic compounds for their anti-angiogenic properties by observing their effects on endothelial cell tube formation.
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Studying Molecular Pathways: Investigate the signaling pathways and molecular mechanisms governing endothelial cell dynamics during angiogenesis.
Choosing Creative Biolabs for Your Cell Tube Formation Assays
At Creative Biolabs, we are dedicated to advancing scientific discovery through innovative assay development. Whether you are exploring the fundamentals of angiogenesis or seeking new therapeutic targets, our cell tube formation assay services offer the precision and reliability you need. For more information or to discuss your specific requirements, please contact us.
For Research Use Only | Not For Clinical Use
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