CelluTest™ Caspase-3 Assay Kit

CAT#: ITS-1125-WB18
Product Type: Assay Kit
Species: All Species
Target: Caspase-3 (CPP32, apopain, or YAMA), the primary executioner of the apoptotic protease cascade.
Brand: CelluTest
Development Stage: Research-Grade Cell-Based Assay Development
Short Description
Quantifies Caspase-3 activity using a colorimetric method.
Brand
CelluTest
Development Stage
Research-Grade Cell-Based Assay Development
Description
The CelluTest™ Caspase-3 Assay Kit provides a high-throughput solution for quantifying apoptosis execution by measuring caspase-3 activity in cultured cells or lysates. Utilizing a specific fluorogenic substrate (N-Ac-DEVD-AFC), this "mix-incubate-measure" system enables rapid screening of pro-apoptotic or anti-apoptotic drug candidates. By delivering sensitive, real-time kinetic data, it facilitates rigorous efficacy testing and mechanistic studies in pre-clinical pharmaceutical development.
Features
Superior Analytical Sensitivity: Employs advanced fluorometric/colorimetric detection to identify nanomolar variations in enzyme activity across diverse biological matrices.
Validated High-Specificity: Utilizes a proprietary Ac-DEVD-specific substrate, ensuring zero cross-reactivity with other cysteine proteases or non-target caspases.
Streamlined Homogeneous Format: Features a simplified "mix-and-read" protocol compatible with automated systems, significantly reducing hands-on time and experimental error.
Robust Kit Integrity: Optimized buffer systems and stable reagents ensure high signal-to-noise ratios and consistent performance over multiple freeze-thaw cycles.
Process Relevance
Quantifying Caspase-3 activity to validate pro-apoptotic drug efficacy and monitor cellular response to therapeutic stressors during development.
Application Stage
Lead optimization, preclinical safety assessment, and standardized high-throughput screening for anti-cancer compounds.
Applications
Measuring apoptosis in mammalian cell lysates or tissue extracts; ideal for screening caspase inhibitors and evaluating cytotoxic profiles of experimental therapeutics.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Caspase-3 (CPP32, apopain, or YAMA), the primary executioner of the apoptotic protease cascade.
Detection Method
Fluorescence Detection (Ex 400 nm / Em 490 nm)
Sample Type
Cell and tissue lysate
Research Areas
Oncology therapeutics, neurodegenerative diseases, immunology, toxicology, drug discovery (ADME), etc.
Size
100 tests
Data Output Type
Quantitative determination of enzyme activity via kinetic or endpoint absorbance/fluorescence measurement.
Assay Time
60 min
Species
All Species
Storage
Store at −20 °C; upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 ℃ storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
Caspase-3 is a crucial cysteine-aspartic acid protease that coordinates the final stages of apoptosis. Its activation marks the point of no return, leading to systematic protein degradation and cellular disassembly.
Pathways
The enzyme operates downstream of the mitochondrial (intrinsic) and death receptor (extrinsic) pathways. It is activated by Caspase-8 or -9 to cleave vital substrates like PARP.
For Research Use Only | Not For Clinical Use
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