Short Description
Quantifies Caspase-3 activity using a colorimetric method.
Development Stage
Research-Grade Cell-Based Assay Development
Description
The CelluTest™ Caspase-3 Assay Kit provides a high-throughput solution for quantifying apoptosis execution by measuring caspase-3 activity in cultured cells or lysates. Utilizing a specific fluorogenic substrate (N-Ac-DEVD-AFC), this "mix-incubate-measure" system enables rapid screening of pro-apoptotic or anti-apoptotic drug candidates. By delivering sensitive, real-time kinetic data, it facilitates rigorous efficacy testing and mechanistic studies in pre-clinical pharmaceutical development.
Features
Superior Analytical Sensitivity: Employs advanced fluorometric/colorimetric detection to identify nanomolar variations in enzyme activity across diverse biological matrices.
Validated High-Specificity: Utilizes a proprietary Ac-DEVD-specific substrate, ensuring zero cross-reactivity with other cysteine proteases or non-target caspases.
Streamlined Homogeneous Format: Features a simplified "mix-and-read" protocol compatible with automated systems, significantly reducing hands-on time and experimental error.
Robust Kit Integrity: Optimized buffer systems and stable reagents ensure high signal-to-noise ratios and consistent performance over multiple freeze-thaw cycles.
Process Relevance
Quantifying Caspase-3 activity to validate pro-apoptotic drug efficacy and monitor cellular response to therapeutic stressors during development.
Application Stage
Lead optimization, preclinical safety assessment, and standardized high-throughput screening for anti-cancer compounds.
Applications
Measuring apoptosis in mammalian cell lysates or tissue extracts; ideal for screening caspase inhibitors and evaluating cytotoxic profiles of experimental therapeutics.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Caspase-3 (CPP32, apopain, or YAMA), the primary executioner of the apoptotic protease cascade.
Detection Method
Fluorescence Detection (Ex 400 nm / Em 490 nm)
Sample Type
Cell and tissue lysate
Research Areas
Oncology therapeutics, neurodegenerative diseases, immunology, toxicology, drug discovery (ADME), etc.
Datasheet