CelluTest™ Parallel Artificial Membrane Permeability Assay (PAMPA) Kit

CAT#: ITS-1125-WB15
Product Type: Assay Kit
Species: All Species
Target: Small molecule chemical compounds and pharmaceutical candidates utilizing passive transcellular diffusion for biological membrane transport.
Brand: CelluTest
Development Stage: Research-Grade Cell-Based Assay Development
Short Description
Quantifies compound permeability through an artificial gastrointestinal membrane using a spectrophotometric method.
Brand
CelluTest
Development Stage
Research-Grade Cell-Based Assay Development
Description
The CelluTest™ Parallel Artificial Membrane Permeability Assay (PAMPA) Kit offers a cost-effective, high-throughput solution for evaluating the gastrointestinal permeability of drug candidates. This system mimics passive diffusion across biological membranes, providing essential absorption data in early-stage drug discovery. By enabling rapid screening of thousands of compounds, it accelerates lead optimization and enhances predictive accuracy in pharmaceutical development.
Features
Rapid High-Throughput Efficiency: Streamline lead optimization by evaluating thousands of compounds daily using automated 96-well plate formats for maximum productivity.
Cost-Effective Passive Screening: Achieve reliable permeability rankings at a fraction of the cost and time required for labor-intensive cell-based assays.
Versatile Membrane Customization: Adapt lipid compositions to accurately simulate gastrointestinal, blood-brain barrier, or skin environments for diverse tissue-specific penetration studies.
Broad pH Compatibility: Assess ionizable compound stability and absorption kinetics across a wide physiological pH range to predict regional intestinal uptake.
Process Relevance
Predicting passive diffusion rates to identify poorly permeable leads early, preventing costly late-stage clinical attrition and developmental delays.
Application Stage
Early-stage drug discovery, lead optimization, and high-throughput ADME profiling during preclinical development for candidate ranking and selection.
Applications
This kit facilitates gastrointestinal absorption modeling, blood-brain barrier penetration studies, and skin permeability assays for compound ranking, excipient research, and passive diffusion kinetic analysis.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Small molecule chemical compounds and pharmaceutical candidates utilizing passive transcellular diffusion for biological membrane transport.
Detection Method
PAMPA
Sample Type
Chemical compounds
Research Areas
Pharmacokinetics (ADME), transdermal drug delivery, neuropharmacology, gastrointestinal therapeutics development, etc.
Size
96 tests
Data Output Type
Quantitative effective permeability coefficient calculation via UV-Vis absorbance or LC-MS/MS analysis of acceptor and donor concentrations.
Assay Time
20 h
Species
All Species
Storage
Store at −20 °C; upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 ℃ storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
Membrane permeability dictates the systemic bioavailability and target-site concentration of pharmaceutical agents. PAMPA simulates the lipid bilayer to evaluate the passive transcellular diffusion essential for overcoming biological barriers like the intestinal epithelium.
Pathways
This assay models the passive diffusion pathway, the primary mechanism for over 90% of commercial drugs. It bypasses active transport complexities to isolate and rank compounds based purely on membrane partition coefficients.
For Research Use Only | Not For Clinical Use
Online Inquiry