CelluTest™ Succinate Assay Kit

CAT#: ITS-1125-HM46
Product Type: Assay Kit
Species: All Species
Brand: CelluTest
Development Stage: Research-Grade Cellular Function Assay Development
Short Description
Measures succinate levels, a key TCA cycle intermediate.
Brand
CelluTest
Development Stage
Research-Grade Cellular Function Assay Development
Description
The CelluTest™ Succinate Assay Kit measures succinate levels to evaluate mitochondrial Complex II function and TCA cycle integrity. This assay is essential for studying ischemia-reperfusion injury and oncometabolite accumulation in cancer research. By detecting succinate accumulation, it aids in identifying metabolic bottlenecks and validating drugs that target mitochondrial respiration.
Features
Direct measurement without separation steps; Validates mitochondrial toxicity and complex II inhibition.
Process Relevance
Mitochondrial Toxicity Assessment
Application Stage
Discovery / Toxicology
Applications
Investigation of mitochondrial complex II function and hypoxia.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 570 nm, Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Succinate in food, beverage, agricultural products, and other biological samples
Research Areas
Oncology; Metabolic Disease
Size
100 tests
Data Output Type
Quantitative (Concentration)
Assay Time
Results in 30 min
LOD
10 µM (Colorimetric assay, OD); 2 µM (Fluorimetric assay, FL)
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Succinate is a TCA cycle intermediate oxidized by Complex II. Its accumulation during ischemia or in cancer cells with SDH mutations promotes angiogenesis and metabolic adaptation by stabilizing HIF-1alpha.
For Research Use Only | Not For Clinical Use
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