Short Description
Measures succinate levels, a key TCA cycle intermediate.
Development Stage
Research-Grade Cellular Function Assay Development
Description
The CelluTest™ Succinate Assay Kit measures succinate levels to evaluate mitochondrial Complex II function and TCA cycle integrity. This assay is essential for studying ischemia-reperfusion injury and oncometabolite accumulation in cancer research. By detecting succinate accumulation, it aids in identifying metabolic bottlenecks and validating drugs that target mitochondrial respiration.
Features
Direct measurement without separation steps; Validates mitochondrial toxicity and complex II inhibition.
Process Relevance
Mitochondrial Toxicity Assessment
Application Stage
Discovery / Toxicology
Applications
Investigation of mitochondrial complex II function and hypoxia.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 570 nm, Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Succinate in food, beverage, agricultural products, and other biological samples
Research Areas
Oncology; Metabolic Disease
Datasheet