Kit Overview
The IHC detection kit contains all the components needed to detect target protien present in formalin-fixed, paraffin-embedded (FFPE) tissue samples. Creative Biolabs offers the complete and cusom IHC kit to choose from, giving you the ultimate flexibility in planning your Immunohistochemistry protocol.
Chromogenic IHC Kit
Antigen Retrieval Buffer
Protein Blocking Buffer
Anti-CFLAR / FLIP IHC Primary Antibody
Antibody Diluent Solution
HRP/DAB (ABC) IHC Detection Kit*
Chromogenic Counterstains#
DoubleStain IHC Kit (Optional)&
Special Stain Kit (Specified)%
Mounting Medium for IHC
* A complete kit to detect a Primary Antibody
Antibody Diluent Solution using HRP-labeled-streptavidin and a biotinylated secondary antibody.
# Chromogenic counterstains are used when the Primary Antibody
Antibody Diluent Solution is visualized using HRP or alkaline phospatase combined with DAB, AEC, or similar enzyme substrates. The traditional combination is HRP/DAB with hematoxylin to stain nuclei. Eosin, for cytoplasmic staining, is often used in combination with hematoyxolin.
& DoubleStain is designed for user to detect two distinct antigens on human tissue.
% Special Stain Kit are used to stain certain cell types, microorganisms, and specific proteins, carbohydrates and metabolites found in the tissue matrix and within cells.
Fluorescent IHC Kit
Antigen Retrieval Buffer
Protein Blocking Buffer
Anti-CFLAR / FLIP IHC Primary Antibody
Antibody Diluent Solution
Secondary Antibody, Fluorescent-labeled*
Fluorescent Counterstains #
DoubleStain IHC Kit (Optional)&
Special Stain Kit (Specified)%
Mounting Medium for IHC
* Fluorescent labels include FITC, R-PE, Alexa-Fluor®.
# Fluorescent counterstains are used when the Primary Antibody
Antibody Diluent Solution staining is visualized with a fluorescent dye either directly conjugated to the Primary Antibody
Antibody Diluent Solution, or to a secondary antibody.
& DoubleStain is designed for user to detect two distinct antigens on human tissue.
% Special Stain Kit are used to stain certain cell types, microorganisms, and specific proteins, carbohydrates and metabolites found in the tissue matrix and within cells.
Multiplex IHC Detection Kit
Superior multiplex IHC kit is designed for efficient detection of multiple antigens in a single tissue sample.
- Biotin-free nanopolymer-based system for superior multiplex antigen detection
- Complete kit includes for sensitive, crisp 2-color development
- Preserves precious tissue while reducing reagent and labor costs
- Expandable with additional chromogens (sold separately)
IHC Antibody Notes
The anti-CFLAR / FLIP antibody included in the kit is extensively tested and validated for immunohistochemistry applications on human or animal tissues using standard detection methods and procedures-resulting in low background signal, high detection sensitivities, and no cross-reactivity issues.
Use our directly conjugated primary antibodies to speed up and simplify the IHC protocol as they omit the need for a secondary antibody staining step. Our primary conjugates are recombinantly engineered to provide high specificity and batch-to-batch consistency. If your antibody of choice is not available in a suitable conjugated format, you can
Use our antibody conjugation kits.