Chicken IFNA ELISA Kit, Lot 21AO-342 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Chicken IFNA in Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate.

Chicken IFN alpha ELISA Kit.

ELISA

IFNA

Chicken

Colorimetric

Sandwich ELISA

Quantitative

Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

This ELISA antibody pair recognizes Chicken IFN alpha.

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Distilled or deionized water
Precision pipettes to deliver 2 μL to 1 μL volumes
Adjustable 1-25 μL pipettes for reagent preparation
100 μL and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Pre-coated

4 °C

6 months

For Research Use only

Interferon alpha

IFN-ALPHA-1; IFN1; Ifa; Ifa8; interferon; alpha 1; interferon alpha; interferon-alpha; IFNA1; Ifna; IFNA

100857403, 100858506

B0FLN7

JAK-STAT Signaling, TLR Signaling, Hepatitis C, Inflammasome

1.Prepare all reagents, samples and standards as instructed in the manual.
2.Add 100 μL of standard or sample to each well.
3.Incubate 2.5 h at RT or O/N at 4°C.
4.Add 100 μL of prepared biotin antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 μL of prepared Streptavidin solution to each well.
7.Incubate 45 min at RT.
8.Add 100 μL of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 μL of Stop Solution to each well.
11.Read at 450 nm immediately.